Synthesis and biological evaluation of pyrido [4,3-d] pyrimidine analogues as a new class of anti-inflammatory agents

Abstract

The Synthesis of pyrido pyrimidine and its derivative is of high interest in organic chemistry because some possess biological and pharmacological activities. The pyrido pyrimidine functionality has also been used for the development of biologically interesting molecules. Earlier reported procedures for the Synthesis of pyrido pyrimidines typically involved a multistep approach. Pyrido pyrimidines were reported to be used as a cytotoxic agent, mycobacterium dihydrofolate reductase, antitumor, and antiviral and antimicrobial activities. Our interest in the Synthesis and biological evaluation of heterocyclic bicycles and the fact that some of these compounds are not frequently used (pyrido[4,3-d]pyrimidines or are totally absent in commercial compound libraries (e.g., thiazolo pyrimidines and oxazolo pyrimidines), prompted us to elaborate this type of chemistry and to synthesize pyrido[4,3-d]pyrimine heterocyclic scaffolds. A total of three novel pyrido pyrimidine derivatives compounds were synthesized by taking 4,6-diamino-2-Bromo-3-cyanopyridine as starting compounds and were characterized by different analytical techniques like IR, NMR, and Mass spectroscopy. The synthesized compounds were screened for their anti-inflammatory potency in vivo by a suitable animal model.