Simultaneous Estimation of Antidiabetic Drugs in API and Formulations by RP-HPLC Method

Abstract

A straight forward, Exact, exact technique was created for the concurrent assessment of the Remogliflozin and Vildagliptin in drug measurement’s structure. Chromatogram was gone through Phenomenon x C 18 section (150x4.6mm, 5µm). Portable stage containing Phosphate cushion, Acetonitrile and Methanol in the proportion of 30:05:65 was siphoned through section at a stream pace of 1.2 ml/min. Support utilized at pH 4.6. Temperature was kept up with at Encompassing. Upgraded frequency for Remogliflozin and Vildagliptin was 249 nm. Maintenance season of Remogliflozin and Vildagliptin were viewed as 2.102 min and 3.246 min. The % virtue of Remogliflozin and Vildagliptin was viewed as 100.348% and 100.049% separately. The framework appropriateness boundaries for Remogliflozin and Vildagliptin, for example, hypothetical plates and following component were viewed as 3569.028,4798.716, 1.27 and 1.11. The linearity study for Remogliflozin and Vildagliptin relationship coefficient (r2) was viewed as 0.999 and 0.999, % mean recuperation was viewed as 99.03 % and 100.19 %, %RSD for repeatability was 0.944 and 0.548, % RSD for middle of the road accuracy was 0.119 and 0.649 individually. The accuracy study was exact, strong and repeatable. LOD esteem was 0.35 and 0.08, and LOQ esteem was 1.08 and 0.25 separately. The consequences of study showed that the proposed RP‐HPLC technique is a straightforward, exact, exact, rough, hearty, quick and reproducible, which might be helpful for the normal assessment of Remogliflozin and Vildagliptin in drug measurement structure.