Concurrent Determination of Metronidazole And Fenbendazole In Pharmaceutical Dosage Form By Rp-Hplc

Abstract

A simple, accurate method was developed for the simultaneous quantification of fenbendazole (FBZ) and metronidazole (MTZ) in bulk and tablet formulation. The chromatogram was obtained using a Phenomenex, Luna C18, 250 × 4.6 mm, 5 mm column with mobile phase 10 Mm KH2PO4 (pH 4) and methanol (70:30 v/v), with a flow rate of 0.8 ml/min at 305 nm and 30°C column temperature. The retention times for FBZ and MTZ were 3.689 and 7.232 minutes, respectively, and the regression coefficients for FBZ and MTZ were 0.9923 and 0.9918. The percentage recovery was 101.97% and 100.03% for FBZ and MTZ, respectively, and the LOD and LOQ values obtained from the FBZ and MTZ regression equations were 0.08, 0.25 and 0.04, 0.12 respectively. The proposed method was simple, robust, unique, and profitable, making it suitable for veterinary laboratories.