Rp Hplc Method Designing And Assessment To Analyze Albendazole And Closantel Amine In Its Formulation

Abstract

A simple, precise, and accurate method was developed to estimate ALB and CLS in bulk and tablet dosages. Chromatogram was produced using Agilent Zorbax C18 250 x 4.6 mm, 5mm column and 50:50 v/v Acetonitrile and KH2PO4 mobile phase at 0.8 ml/min at 220nm at 30°C column temperature. The retention times for ALB and CLS are 2.189 and 3.079 minutes. The ALB and CLS regression coefficients were 0.9917 and 0.9984. % We recovered 100.14% and 99.98% for ALB and CLS. The ALB and CLS regression equations yielded LOD and LOQ values of 0.08, 0.25, and 0.04, 0.12. Due to reduced retention and run times, the recommended method was simple and cost-effective for routine quality control laboratory testing.