Method Designing And Its Assessment To Determine Cefotaxime And Sulfamethoxazole In Combined Formulation By Hplc

Abstract

A simple, accurate method is used to determine Cefotaxime (CFT) and Sulfamethoxazole (SMZ) in bulk and their combination formulation. Chromatogram was produced on Thermofischer BDS Hypersil C18, 250 x 4.6 mm, 5mm column, with 0.1%formic acid and methanol in 80:20v/v mobile phase, 0.8ml/min flow rate, 265nm, 30°C column CFT and SMZ retention times are 3.453 and 7.991min. We found 0.9957 and 0.9933 regression coefficients for CFT and SMZ. % Recovery was 100.08% for CFT and 100.06% for SMZ. The CFT and SMZ regression models yield LOD values of 0.08, 0.25 and LOQ values of 0.04, 0.12. Simple and cost-effective quality analysis of this combination during clinical trials was suggested.