Development and Validation of a Spectrophotometric method using Vierordt’s Method for Simultaneous Estimation of Moxifloxacin and Cefixime in tablet formulation

Abstract

Objective: The objective of the current study was to develop rapid, accurate, reproducible, validated and economical
Vierordt’s Method for the simultaneous determination of CEF and MOX in tablet dosage forms. Method: This
method of analysis was based upon the absorption of drugs at wavelength maximum of each other. Two
wavelengths of 289.10 and 295.10 nm were selected which are the λmax of two drugs for the development of the
simultaneous equations. The absorbance of CEF and MOX were measured and the absorptivity values were
determined at all the two selected wavelengths. Result & Discussion: The linearity was found to be 3-9μg/ml for
CEF & MOX respectively. Recovery was in the range of 98 –102%; the values of standard deviation and% R.S.D.
were found to be <2% shows the high accuracy of the method. The Limit of Detection and Limit of Quantitation
were theoretically calculated which were found to be 0.0224 and 0.0678 for CEF and 0.0070 and 0.0214 for MOX
respectively. Robustness and Ruggedness were also carried out and percentage RSD was found to be less than 2.0
%. The assay of Cefixime and Moxifloxacin was found to be 99.36% and 98.75%.The proposed method has been
validated as per ICH guidelines and successfully applied to the estimation of CEF and MOX in their combined
Tablet dosage form.